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KMID : 1145120170150040457
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 4 p.457 ~ p.465
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Anti-oxidant Activities of Phytol on Keratinocytes
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Jeong Sun-Hee
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Abstract
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Purpose: The aim purpose of this study is to evaluate anti-oxidant and cytoprotective effects of phytol as an ingredient of cosmetic against hydrogen peroxide (H2O2) in keratinocytes.
Methods: Cell viability assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2’,7’-dichlorofluorescin diacetate (DCFDA) assay, reactive oxygen species (ROS) scavenging activity assay, flow cytometry analysis, quantitative real-time polymerase chain reaction (qRT-PCR), and glutathione assay were performed to verify the cell effectiveness of phytol.
Results: To determine the experimental concentration of H2O2 and phytol in HaCaT keratinocytes, water-soluble tetrazolium salt (WST-1) assay was examined with treatments of various H2O2 and phytol concentrations in HaCaT keratinocytes. As a results 750 μM H2O2 and 10 μM phytol treatment was chosen for the experiment. The expression of copper-zinc superoxide dismutase (CuZn-SOD; SOD1), manganese superoxide dismutase (Mn-SOD; SOD2), catalase (CAT) mRNA, very well-known as anti-oxidant genes, was increased by phytol in a dose-dependent manner. DPPH radical scavenging activity and intracellular reduced form of glutathione (GSH) levels were increased by phytol in a dose-dependent manner, which showed that anti-oxidant effects are recovered by phytol.
Conclusion: It suggests possibility of phytol as a cosmetic ingredient which enhances a defense system and slows an ageing process for skin.
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KEYWORD
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Phytol, Anti-oxidant, Cytoprotective, Cosmetics, Ageing
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